Composite

Part:BBa_K3102037:Design

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)


T7-RBS-PFLB-Ter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 534
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 836


Design Notes

None


Source

This sequence comes from Shewanella Oneidensis and was found through Biocyc: https://biocyc.org/gene?orgid=SONE211586&id=G1GMP-2686

Promoter (BBa_I719005), RBS (BBa_B0034) and Terminator (BBa_B0015) are from the iGEM Registry.

References

Li F, Li YX, Cao YX, Wang L, Liu CG, Shi L, et al. Modular engineering to increase intracellular NAD(H/+) promotes rate of extracellular electron transfer of Shewanella oneidensis. Nat Commun [Internet]. 2018;9(1):1–13. Available from: http://dx.doi.org/10.1038/s41467-018-05995-8